The adenylate cyclase receptor complex and aqueous humor formation.

The secretory tissue of the eye, the ciliary processes, contains an enzyme receptor complex, composed of membrane proteins, the catalytic moiety of the enzyme adenylate cyclase, a guanyl nucleotide regulatory protein (or N protein), and other features. The enzyme can be activated by well-known neurohumoral or humoral agents, catecholamines, glycoprotein hormones produced by the hypothalamic pituitary axis, and other related compounds, including placental gonadotropin, organic fluorides, and forskolin, a diterpene. These compounds cause the ciliary epithelia to produce cyclic AMP at an accelerated rate. Cyclic AMP, as a second messenger, causes, either directly or indirectly, a decrease in the net rate of aqueous humor inflow that may be modulated by cofactors. Clinical syndromes fit the experimental data so that an integrated explanation can be given for the reduced intraocular pressure witnessed under certain central nervous system and adrenergic influences. The molecular biology of this concept provides important leads for future investigations that bear directly both upon the regulation of intraocular pressure and upon glaucoma

EXPERIENCES OF UAV SURVEYS APPLIED TO ENVIRONMENTAL RISK MANAGEMENT

In this paper the results of some surveys carried out in an area of Apulian territory affected by serious environmental hazard are presented. Unmanned Aerial Vehicles (UAV) are emerging as a key engineering tool for future environmental survey tasks. UAVs are increasingly seen as an attractive low-cost alternative or supplement to aerial and terrestrial photogrammetry due to their low cost, flexibility, availability and readiness for duty. In addition, UAVs can be operated in hazardous or temporarily inaccessible locations, that makes them very suitable for the assessment and management of environmental risk conditions. In order to verify the reliability of these technologies an UAV survey and A LIDAR survey have been carried outalong about 1 km of coast in the Salento peninsula, near the towns of San Foca, Torre dellOrso and SantAndrea( Lecce, Southern Italy). This area is affected by serious environmental risks due to the presence of dangerous rocky cliffs named falesie. The UAV platform was equipped with a photogrammetric measurement system that allowed us to obtain a mobile mapping of the fractured fronts of dangerous rocky cliffs. UAV-images data have been processed using dedicated software (AgisoftPhotoscan). The point clouds obtained from both the UAV and LIDAR surveys have been processed using Cloud Compare software, with the aim of testing the UAV results with respect to the LIDAR ones. The total error obtained was of centimeter-order that is a very satisfactory result. The environmental information has been arranged in an ArcGIS platform in order to assess the risk levels. The possibility to repeat the survey at time intervals more or less close together depending on the measured levels of risk and to compare the output allows following the trend of the dangerous phenomena. In conclusion, for inaccessible locations of dangerous rocky bodies the UAV survey coupled with GIS methodology proved to be a key engineering tool for the management of environmental risks

Role of dorsomedial striatum neuronal ensembles in incubation of methamphetamine craving after voluntary abstinence

Abstract We recently developed a rat model of incubation of methamphetamine craving after choice-based voluntary abstinence. Here, we studied the role of dorsolateral striatum (DLS) and dorsomedial striatum (DMS) in this incubation. We trained rats to self-administer palatable food pellets (6 d, 6 h/d) and methamphetamine (12 d, 6 h/d). We then assessed relapse to methamphetamine seeking under extinction conditions after 1 and 21 abstinence days. Between tests, the rats underwent voluntary abstinence (using a discrete choice procedure between methamphetamine and food; 20 trials/d) for 19 d. We used in situ hybridization to measure the colabeling of the activity marker Fos with Drd1 and Drd2 in DMS and DLS after the tests. Based on the in situ hybridization colabeling results, we tested the causal role of DMS D1 and D2 family receptors, and DMS neuronal ensembles in "incubated" methamphetamine seeking, using selective dopamine receptor antagonists (SCH39166 or raclopride) and the Daun02 chemogenetic inactivation procedure, respectively. Methamphetamine seeking was higher after 21 d of voluntary abstinence than after 1 d (incubation of methamphetamine craving). The incubated response was associated with increased Fos expression in DMS but not in DLS; Fos was colabeled with both Drd1 and Drd2 DMS injections of SCH39166 or raclopride selectively decreased methamphetamine seeking after 21 abstinence days. In Fos-lacZ transgenic rats, selective inactivation of relapse test-activated Fos neurons in DMS on abstinence day 18 decreased incubated methamphetamine seeking on day 21. Results demonstrate a role of DMS dopamine D1 and D2 receptors in the incubation of methamphetamine craving after voluntary abstinence and that DMS neuronal ensembles mediate this incubation. SIGNIFICANCE STATEMENT: In human addicts, abstinence is often self-imposed and relapse can be triggered by exposure to drug-associated cues that induce drug craving. We recently developed a rat model of incubation of methamphetamine craving after choice-based voluntary abstinence. Here, we used classical pharmacology, in situ hybridization, immunohistochemistry, and the Daun02 inactivation procedure to demonstrate a critical role of dorsomedial striatum neuronal ensembles in this new form of incubation of drug craving

Combining Revealed and Stated Preferences to design a new urban park in a metropolitan area of North-Western Italy

Inclusive and participatory decision-making is a sustainable option for governments and decision-makers to support real transformation and planning of policies and actions. Investigating and gathering the various views and opinions of stakeholders and citizens is particularly effective because it opens up a range of possibilities in co-constructing the city of the future. Among urban areas requiring planning, Urban Green Infrastructures (UGIs) represent spaces designed to improve the character of neighborhoods, as well as to increase the well-being of users. To achieve these goals, planners should adopt a design approach in which UGIs projects are shaped by local community concerns rather than by market conventions in urban design. Focusing on green recreational areas, this study employs an integrated approach combining Revealed (RP) and Stated Preferences (SP) to investigate citizens' preferences regarding urban parks. In particular, the experiment combines Travel Cost Method (TCM) and Discrete Choice Experiment (DCE) for supporting a requalification project in an ex-industrial area of Turin (Italy). In this way, it was possible to understand which facilities can contribute to increasing the citizens’ well-being and the overall efficiency of the UGIs provision and maintenance. The proposed methodology represents an operational and replicable procedure to support different renewal projects in which citizens' opinions are crucial for developing long-term sustainable socio-ecological plans and actions

The influence of different types of preparation (espresso and brew) on coffee aroma and main bioactive constituents

Coffee is one of the most popular hot drinks in the world; it may be prepared by several methods, but the most common forms are boiled (brew) and pressurized (espresso). Analytical studies on the substances responsible for the pleasant aroma of roasted coffee have been carried out for more than 100 years. Brew coffee and espresso coffee (EC) have a different and peculiar aroma profile, demonstrating the importance of the brewing process on the final product sensorial quality. Concerning bioactive compounds, the extraction mechanism plays a crucial role. The differences in the composition of coffee brew in chlorogenic acids and caffeine content is the result of the different procedures of coffee preparation. The aim of the present review is to detail how the brewing process affects coffee aroma and composition

Dynamical effects of self-generated magnetic fields in cosmic ray modified shocks

Recent observations of greatly amplified magnetic fields ($\delta B/B\sim 100$) around supernova shocks are consistent with the predictions of the non-linear theory of particle acceleration (NLT), if the field is generated upstream of the shock by cosmic ray induced streaming instability. The high acceleration efficiencies and large shock modifications predicted by NLT need however to be mitigated to confront observations, and this is usually assumed to be accomplished by some form of turbulent heating. We show here that magnetic fields with the strength inferred from observations have an important dynamical role on the shock, and imply a shock modification substantially reduced with respect to the naive unmagnetized case. The effect appears as soon as the pressure in the turbulent magnetic field becomes comparable with the pressure of the thermal gas. The relative importance of this unavoidable effect and of the poorly known turbulent heating is assessed. More specifically we conclude that even in the cases in which turbulent heating may be of some importance, the dynamical reaction of the field cannot be neglected, as instead is usually done in most current calculations.Comment: 4 pages, 1 figure, accepted for publication in ApJ Letter

The reintroduction of Castor fiber in Piedmont (Italy): An integrated SWOT-spatial multicriteria based approach for the analysis of suitability scenarios

The Castor fiber or Eurasian beaver can change its habitat by building dams and creating ponds. For this reason, Castor fiber is known as an “ecosystem engineer” for aquatic and riparian environments. Despite its ecological importance, at the beginning of the 20th century the population was reduced to only 1200 beavers in Europe and Asia, due to uncontrolled hunting. Recently, some reintroductions and translocations have partly re-established the population. In Italy, however, the beaver disappeared in the 16th century and no action has been taken despite the recommendation of the Council of Europe to perform a feasibility study. This research evaluates beaver reintroduction and identifies suitable areas in Italy and, in particular, in the Piedmont region. In order to achieve this, a SWOT analysis combined with a Spatial Multicriteria Analysis was performed. Firstly, the zoological and ethological aspects concerning this rodent were studied, as well as the historical reasons that led to its disappearance in Italy and near extinction in Eurasia. Secondly, Strengths, Weaknesses, Opportunities and Threats (SWOT) of the territory were identified for beaver reintroduction. The SWOT analysis was implemented, as the starting point for the spatial multicriteria analysis. Thirdly, the Multicriteria Spatial Decision Support System (MC-SDSS) was structured into two criteria, i.e. Potentials and Criticalities, representing the spatialization of strengths and weaknesses. The final result of the MC-SDSS is a map showing suitable areas for beaver reintroduction in Piedmont. This map is the weighted sum of the maps of criticalities and potentialities, performed through a set of GIS operations and weighted through a pairwise comparison of criteria by experts. The analysis was conducted for the Piedmont region, but the integrated approach and the set of criteria can also be applied in other regions. Moreover, this mixed-method approach takes into account the characteristics necessary for the choice of suitable beaver habitats and also includes economic and social aspects. Therefore, it is an improvement on the Habitat Suitability Index (HIS), generally used in reintroductions. The aspects considered in the analysis are fundamental for the future development of a shared action plan, which considers both technical and social motivations and acts for the long-term on a wide area

Antibiotic resistance patterns of faecal indicator organisms and occurrence of Salmonella spp. in wild boar (Sus scrofa scrofa) in Italy.

In order to monitor antibiotic resistance in faecal indicator organisms and evaluate the occurrence of Salmonella spp., faeces from 110 wild boars (Sus scrota scrota), killed during a demographic control program in two different regional parks in Bologna province, were collected from September 2002 to June 2003. A single isolate of Escherichia coli, Enterococcus faecalis and Enterococcus faecium from each sample was tested for antibiotic susceptibility using the agar diffusion method recommended by CLSI (formerly, NCCLS). A total of 110 E. coli, 48 E. faecium and 5 E. faecalis strains were isolated and submitted to antibiotic susceptibility tests. Antibiotic resistance patterns were similar in wild boar populations from both parks. Multiple antibiotic-resistance to ciprofloxacin, rifampin and erythromycin was found with h1gh frequency in Enterococcus spp strains. E. coli isolates showed a low antibiotic resistance level. Two Salmonella arizonae and one Salmonella spp. strains, isolated from wild boars of one park, didn\u27t show any resistance concerning the antibiotics tested

Identification of Dimethyldioctadecylammonium Ion (m/z 550.6) and Related Species (m/z 522.6, 494.6) as a Source of Contamination in Mass Spectrometry

Chemical contamination can be one of the more common problems encountered when performing trace-level analysis regardless of the analytical technique. Minimizing or eliminating background interferences can be a difficult task, so knowledge of the chemical composition of these contaminants can prove invaluable when it comes to identifying the source. Once the source is identified, proper steps may be taken to reduce or eliminate it. In this study, we report the identity of some commonly seen contaminants (m/z 550.6, 522.6, and 494.6) in electrospray ionization (ESI) mass spectrometry (MS). Through MS, tandem MS, accurate-mass, and high-resolution measurements we have identified these background contaminants as being quaternary ammonium species that contain long-chain hydrocarbon groups, where m/z 550.6 is a dimethyldioctadecylammonium ion (C18, C18) and m/z 522.6 and 494.6 are similar in nature but have shorter alkyl-chain groups. The lipophilic nature of these compounds and the fact that they have molecular weights similar to lysophospholipids make them a frequent contaminant in lipidomic studies. The likely sources of these compounds are commonly used personal and household products